RNA-Seq variant calling is a strong method for finding genetic changes in regions of the genome that are being actively transcribed. This paper outlines a clear workflow for finding germline and somatic mutations in RNA-sequencing data. It includes key steps like quality control, alignment, variant detection, filtering, and final validation. We examine cutting-edge computational tools... https://www.cd-genomics.com/resource-rna-seq-variant-calling-pipeline-transcript-mutations.html